RP-HPLC-PDA-ESI-MS/MS screening of bioactive compounds from Degla-Beida dates: Conventional and green extraction technologies

  • Kahina Djaoud Laboratoire de Biomathématiques, Biophysique, Biochimie, et Scientométrie, Faculté des Sciences de la Nature et de la Vie, Université de Bejaia
  • Maria Daglia Department of Drug Sciences, Medicinal Chemistry and Pharmaceutical Technology Section, University of Pavia
  • Arold J. T. Sokeng Department of Drug Sciences, Medicinal Chemistry and Pharmaceutical Technology Section, University of Pavia
  • Ferriel Kermiche Laboratoire de Biomathématiques, Biophysique, Biochimie, et Scientométrie, Faculté des Sciences de la Nature et de la Vie
  • Lynda Arkoub Laboratoire de Biomathématiques, Biophysique, Biochimie, et Scientométrie, Faculté des Sciences de la Nature et de la Vie
  • Khodir Madani Laboratoire de Biomathématiques, Biophysique, Biochimie, et Scientométrie, Faculté des Sciences de la Nature et de la Vie
  • Lila Boulekbache Makhlouf Laboratoire de Biomathématiques, Biophysique, Biochimie, et Scientométrie, Faculté des Sciences de la Nature et de la Vie
Keywords: microwave-assisted extraction (MAE), phenolic compounds, optimization, antioxidant activity, HPLC-PAD-ESI-MS/MS analysis

Abstract

Date fruits have vital therapeutic properties, in the way that they represent an inexhaustible source of bioactive compounds such as polyphenols. Therefore, the objective of this work was to evaluate the phytocomposition by High Performance Liquid Chromatography (RP-HPLC-PAD-ESI-MS/MS) of the optimized microwaveassisted extraction (MAE) phenolic extract by using the response surface methodology (RSM) compared to the ultrasound-assisted and conventional extractions (UAE and CE), and to test its antioxidant activity in vitro. The resulting regression model indicated that a quadratic polynomial model was best suited for the spectrophotometrically determined total phenolics. The maximum total phenolic content, TPC, (4.27±0.09 mg GAE/g DW) was obtained with a 50% (v/v) ethanol’s concentration, a 700 W microwave power and an extraction time of 2.42 minutes. HPLC analysis revealed the presence of organic sugars and acids, nucleoside, carotenoids, proanthocyanidins, lignans, flavonols, flavones, saponin and sterol glucosides. The MAE phenolic extract showed 58.72±0.29% DPPH radical scavenging activity and a 1.88±0.09 mg AscAE/mL reducing power.

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Published
2020-06-26
How to Cite
Djaoud, Kahina, Maria Daglia, Arold Sokeng, Ferriel Kermiche, Lynda Arkoub, Khodir Madani, and Lila Makhlouf. 2020. “RP-HPLC-PDA-ESI-MS/MS Screening of Bioactive Compounds from Degla-Beida Dates: Conventional and Green Extraction Technologies”. The Annals of the University Dunarea De Jos of Galati. Fascicle VI - Food Technology 44 (1), 58-81. https://doi.org/https://doi.org/10.35219/foodtechnology.2020.1.04.
Section
ORIGINAL ARTICLES