Application of box-behnken design for optimizing the process of microencapsulation of Bifidobacterium bifidum Bb28

Abstract

The three-level Box-Behnken design, combined with the analysis of variance (ANOVA), was employed in the present study to optimize the process of microencapsulation of Bifidobacterium bifidum BB28. The optimization was based on the encapsulation yield. The results showed that the encapsulation yield could be enhanced significantly when the mixture ratio of cell suspension-alginate
was 1:11.5, sodium alginate 1.9%, and sodium ascorbate 0.065%. The optimal encapsulation yield of Bifidobacterium bifidum BB28 reached 91.52%. The experimental result in terms of encapsulation yield under optimal conditions was very close to the expected value of 91.97%. Therefore, the optimal conditions for encapsulating Bifidobacterium bifidum BB28 were accurately predicted through
statistical methods.