A spectroscopic study on the heat induced changes of glucose oxidase at acidic pH values

  • Loredana Dumitrașcu Dunarea de Jos University, Galati
  • Nicoleta Stănciuc Dunarea de Jos University, Galati
  • Iuliana Aprodu Dunarea de Jos University, Galati
  • Gabriela Bahrim Dunarea de Jos University, Galati
Keywords: glucose oxidase, fluorescence, heat treatment, quenching

Abstract

The heat induced conformational and structural changes of glucose oxidase from Aspergillus niger at pH 5.0 and 4.0 were investigated using fluorescence spectroscopy. Experimental studies were conducted in buffer solution in the 25°C- 70°C temperature range at constant time. At both pH values, the phase diagram was linear, indicating the presence of two molecular species induced by thermal treatment. When thermally treating the glucose oxidase at pH 4.0, the denaturation process was faster than the one at pH 5.0, probably due to the release of cofactor FAD and molten globule formation. The quenching experiments using acrylamide and iodide revealed a more flexible conformation of glucose oxidase at higher temperatures, especially at pH 4.0.

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Published
2014-11-20
How to Cite
Dumitrașcu, Loredana, Nicoleta Stănciuc, Iuliana Aprodu, and Gabriela Bahrim. 2014. “A Spectroscopic Study on the Heat Induced Changes of Glucose Oxidase at Acidic PH Values”. The Annals of the University Dunarea De Jos of Galati. Fascicle VI - Food Technology 38 (2), 81-93. https://www.gup.ugal.ro/ugaljournals/index.php/food/article/view/1778.
Section
ORIGINAL ARTICLES