Emphasizing the Toxicity of Heavy Metal Ions on Plants: Monitoring with an Amino Acids Assay
Keywords:
biostructure, determining amino acids, hypoxia, germination tests, heavy metals, ninhydrin reagent
Abstract
In order to emphasize modifications on living organisms resulted from interaction with heavy metals, we applied a simple, sensitive, quick, inexpensive and reproducible method. It evaluates modifications through determining released amino acids. This method comprises three stages: treating plants with the investigated heavy metals, extracting resulted amino acids and dosing them with a ninhydrin reagent. The colored solution resulted from ninhydrin dosing is spectrophotometrically measured at 516 nm. The Lambert-Beer law is valid within the concentration field of 0-20 μg mL–1.
.
References
[1]. Sarkar, A., Ray, D., Shrivastava, A., Sarker, S. - Molecular Biomarkers: their significance and application in marine pollution monitoring, Ecotoxicology. 15(8): 333-340; (2006).
[2]. Macovschi E. - Biostructura, Ed. Academiei, Bucharest, p. 190, (1969).
[3]. Bell, F. G., Bullock, S. E. T., Halbich, T. F. J., Environmental impacts associated with an abandoned mine in the Witbank coalfield, South Africa. Int. J. Coal Geol., 45, pp. 195-216, (2001).
[4]. Passariello, B., Giuliano, V., Quaresima, S., Barbaro, M., Caroli, S., Forte, G., Garelli, G., Iavicoli, I. - Evaluation of the environmental contamination at an abandoned mining site, Microchemical Journal, 73, pp. 245–50, (2002).
[5]. Gimeno-Garcia, E., Andrue, V., Boluda, R. - Heavy metals incidence in the application of inorganic fertilizers and pesticides to rice farming soils, Environmental Pollution, 92, pp. 19–25, (1996).
[6]. Wojtaszek, P. - Oxidative burst: an early plant response to pathogen infection, Biochemical Journal 322, pp. 681–92, (1997).
[7]. Yadav, S. K. - Heavy metals toxicity in plants: An overview on the role of glutathione and phytochelatins in heavy metal stress tolerance of plants, South African Journal of Botany, 76(2), p. 167-79 (2010).
[8]. E. Macovschi, V. Elefterescu, I. Cornoiu, C. Muşolan, O. Gozia - St. cerc. Biochim., 15, pp. 377-389, (1972).
[9]. Hunter, D., Russell, D. S. - Biopolymers (Peptide Science), 84, pp. 283 – 297 (2006).
[10]. Mithofer, A., Schulze, B., Boland - Biotic and heavy metal stress response in plants: evidence for common signals, W. FEBS Letters, 566, pp. 1–5, (2004).
[11]. Zhou, Z. S., Huang, S. Q., Guo, K., Mehta, S. K., Zhang, P. C., Yang, Z. M. - Metabolic adaptations to mercury-induced oxidative stress in roots of Medicago sativa L, Journal of Inorganic Biochemistry 101, pp. 1–9, (2007).
[12]. Reddy, A. M., Kumar, S. G., Jyonthsnakumari, G., Thimmanaik, S., Sudhakar, C. - Lead induced changes in antioxidant metabolism of horsegram (Macrotyloma uniflorum (Lam.) Verdc.) and bengalgram (Cicer arietinum L.), Chemosphere, 60, p. 97-104, (2005).
[13]. Pandey, N., Sharma, C. P. - Effect of heavy metals Co2+, Ni2+ and Cd2+ on growth and metabolism of cabbage, Plant Science, 163, pp. 753–58, (2002).
[14]. Drochioiu, G., Sunel, V., Oniscu, C., Basu, C., Murariu, M. - Roum. Biotechnol. Lett., 6(2), pp. 155-165, (2001).
[2]. Macovschi E. - Biostructura, Ed. Academiei, Bucharest, p. 190, (1969).
[3]. Bell, F. G., Bullock, S. E. T., Halbich, T. F. J., Environmental impacts associated with an abandoned mine in the Witbank coalfield, South Africa. Int. J. Coal Geol., 45, pp. 195-216, (2001).
[4]. Passariello, B., Giuliano, V., Quaresima, S., Barbaro, M., Caroli, S., Forte, G., Garelli, G., Iavicoli, I. - Evaluation of the environmental contamination at an abandoned mining site, Microchemical Journal, 73, pp. 245–50, (2002).
[5]. Gimeno-Garcia, E., Andrue, V., Boluda, R. - Heavy metals incidence in the application of inorganic fertilizers and pesticides to rice farming soils, Environmental Pollution, 92, pp. 19–25, (1996).
[6]. Wojtaszek, P. - Oxidative burst: an early plant response to pathogen infection, Biochemical Journal 322, pp. 681–92, (1997).
[7]. Yadav, S. K. - Heavy metals toxicity in plants: An overview on the role of glutathione and phytochelatins in heavy metal stress tolerance of plants, South African Journal of Botany, 76(2), p. 167-79 (2010).
[8]. E. Macovschi, V. Elefterescu, I. Cornoiu, C. Muşolan, O. Gozia - St. cerc. Biochim., 15, pp. 377-389, (1972).
[9]. Hunter, D., Russell, D. S. - Biopolymers (Peptide Science), 84, pp. 283 – 297 (2006).
[10]. Mithofer, A., Schulze, B., Boland - Biotic and heavy metal stress response in plants: evidence for common signals, W. FEBS Letters, 566, pp. 1–5, (2004).
[11]. Zhou, Z. S., Huang, S. Q., Guo, K., Mehta, S. K., Zhang, P. C., Yang, Z. M. - Metabolic adaptations to mercury-induced oxidative stress in roots of Medicago sativa L, Journal of Inorganic Biochemistry 101, pp. 1–9, (2007).
[12]. Reddy, A. M., Kumar, S. G., Jyonthsnakumari, G., Thimmanaik, S., Sudhakar, C. - Lead induced changes in antioxidant metabolism of horsegram (Macrotyloma uniflorum (Lam.) Verdc.) and bengalgram (Cicer arietinum L.), Chemosphere, 60, p. 97-104, (2005).
[13]. Pandey, N., Sharma, C. P. - Effect of heavy metals Co2+, Ni2+ and Cd2+ on growth and metabolism of cabbage, Plant Science, 163, pp. 753–58, (2002).
[14]. Drochioiu, G., Sunel, V., Oniscu, C., Basu, C., Murariu, M. - Roum. Biotechnol. Lett., 6(2), pp. 155-165, (2001).
Published
2014-03-15
How to Cite
1.
PINTILIE O, ZAHARIA M, TUDORACHI L, BĂNCILĂ S, DROCHIOIU G, SANDU I. Emphasizing the Toxicity of Heavy Metal Ions on Plants: Monitoring with an Amino Acids Assay. The Annals of “Dunarea de Jos” University of Galati. Fascicle IX, Metallurgy and Materials Science [Internet]. 15Mar.2014 [cited 29Apr.2024];37(1):51-6. Available from: https://www.gup.ugal.ro/ugaljournals/index.php/mms/article/view/2647
Issue
Section
Articles
