Anti-candida activity of kluyveromyces lactis CMGB 226 from dairy products

  • Csutak Ortansa University of Bucharest, Romania
  • Cîrpici Ioana University of Bucharest, Romania
  • Ionescu Robertina University of Bucharest, Romania
  • Stoica Ileana University of Bucharest, Romania
  • Vassu Tatiana University of Bucharest, Romania
Keywords: Kluyveromyces, Candida, killer toxin, viability, 5.8S rDNA, RAPD

Abstract

Yeast killer toxins represent an interesting field of study not only for food preservation, but also for development of new bioproducts with biomedical applications. The yeast strain Kluyveromyces lactis CMGB 226 from dairy products was identified using PCR-RFLP on ITS1-5.8S rDNA-ITS2 region. The Hae III and Hinf I restriction profiles of the 675 bp amplicon were similar to those from reference yeast strains. The RAPD analysis using the primer M13 assured the best discriminatory inter and intraspecific characterization of our strain. Killer screening tests were positive against five Candida species, the highest response being observed against C. parapsilosis CBS 604. The 10- fold concentrated killer toxin had a good thermostability and was more active when resuspended in citrate-phosphate buffer with pH from 5.0 to 6.2. The correlation of pH with temperatures of 22, respectively, 28oC represented an important fungistatic factor. Longer treatments with the toxin caused a decline in the viability of C. parapsilosis CBS 604 sensitive cells, with an augmented effect at 28oC after 24 hours. The combination of conditions near to human body favors the killer toxin activity, representing a potential benefit for its future biomedical use against pathogenic Candida strains.

Published
2015-11-12
How to Cite
1.
Ortansa C, Ioana C, Robertina I, Ileana S, Tatiana V. Anti-candida activity of kluyveromyces lactis CMGB 226 from dairy products. Innovative Romanian Food Biotechnology [Internet]. 12Nov.2015 [cited 29Apr.2024];(17):46-. Available from: https://www.gup.ugal.ro/ugaljournals/index.php/IFRB/article/view/3505
Issue
No 17 (2015)
Section
Articles

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