Molecular hybridization techniques of nucleic acids

  • Barbu Vasilica Dunarea de Jos University of Galati
Keywords: blotting, target, probe, primers, renaturation, vectors

Abstract

The nucleic acid hybridization is the process wherein two DNA or RNA single chains (mono-stranded) from different biological sources, make the double catenary configuration, based on contingent sequence homology of the two sources, resulting DNA-DNA, RNA-RNA or DNA-RNA hybrids. The purpose is identification or localization of certain nucleic acid sequences (genes) in thegenome of somespecies. The target molecule representing the DNA, RNA or protein sequence that should be identified or located and the probe molecule who identify the target, by hybridization. Hybridization on a solid carrier is: Southern blotting, Northern blotting, Western blotting. Hybridization stages is: probe synthesis, probe marking (radioactively or non-radioactively), target DNA processing, target DNA denaturation, target DNA transfer to solid carrier, molecular hybridization.

Published
2007-09-28
How to Cite
1.
Vasilica B. Molecular hybridization techniques of nucleic acids. Innovative Romanian Food Biotechnology [Internet]. 28Sep.2007 [cited 4May2024];(1):1-1. Available from: https://www.gup.ugal.ro/ugaljournals/index.php/IFRB/article/view/3285
Issue
No 1 (2007)
Section
Articles

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