Progress in aerobic bioprocess modeling and control. Case study

Abstract

The overview presents the most important and recent issues regarding the modelling and the control of the aerobic bioprocess. First part is dedicated to the discussion about instruments and techniques for bioprocess variables determination and monitoring: (a) the standard direct physical determinations: temperature, pressure (over pressure), agitator shaft power and rate of stirring, foam, gas and liquid flow, weight; (b) the regular chemical determinations: pH, redox potential, dissolved oxygen concentration, exit-gas analysis, on-line analysis of other chemical factors (ion-specific sensors, enzyme electrodes, microbial electrodes, mass spectrometers, fluorimeters). In the second part the mathematical modelling of the aerobic bioprocess is presented with several types of models: unstructured global models; structured models; segregated models; metabolic modelling. The bioprocess control has different goals and objectives, function of bioprocess characteristics and imposed performances. A case study based on authors’ original research presents the bioprocess modelling and the intelligent structure design to control the fed batch cultivation of Hansenula polymorpha CBS-4732 yeast for alcoholoxidase-containing cellular mass.