Identification of potential strain - Buttiauxella izardii DSM 9397 for remediation of cadmium

Abstract

The environmental disposal site in particular heavy metals contaminant has been studied in small scale industries located in Mira Bhayander road, Mumbai, India. The physico-chemical and microbial characterization of the waste has been carried out. Physico-chemical parameters studied found to provide favorable environmental conditions to the adapted microorganism. Microbial consortium assessed showed the presence of Bacillus cereus, Bacillus licheniformis, Cloacibacterium normanense, Bacillus licheniformis, Klebsiela pneumoniae, Buttiauxella izardii, Citrobacter freundii. Cadmium was exposed to microorganism consortium at concentrations such as 5 mg/l, 25 mg/l, 50 mg/l, 100 mg/l up to 800 mg/l to identify the organism which could survive at a higher concentration. Individual colony found was further identified by sequencing and developing phylogenetic tree based on bioinformatics tools – BLAST. Selected strain Buttiauxella izardii DSM 9397, was identified as potential microorganism for remediation of heavy metal- cadmium which will be applicable to decontaminate the environment.