Sulphate Reducing Bacteria in Biofilms on Thermosetting Polymers/Zn Composite Layers

  • Alina CIUBOTARIU "Dunarea de Jos" University of Galati, Romania
  • Lidia BENEA "Dunarea de Jos" University of Galati, Romania
  • Wolfgang SAND University of Duisburg Essen, Biofilm Centre, Aquatic Biotechnology, Germany
Keywords: biofilm, composite layers, Sulphate Reducing Bacteria, Atomic Force Microscopy, Epifluorescence Microscopy, roughness

Abstract

Bacterial adhesion to surfaces is the first step in the formation of a biofilm and has been studied extensively over the past decades in many diverse applications. Sulphate Reducing Bacteria (SRB) is a group of phylogenetically diverse anaerobic microorganisms that were first discovered by Beijerinck in 1895. This work investigates the attachment of Sulphate Reducing Bacteria and the modification of roughness before and after the attachments on the surfaces of zinc and thermosetting polymers/zinc composite layers obtained by electro co-deposition. There were used two types of thermosetting polymers: phenol – formaldehyde resin (type NOVOLAC) and epoxi resin. For investigations of the surfaces were used atomic force and epifluorescence microscopy methods (AFM and EFM, respectively). Sessile bacteria on coupons were stained with 4’, 6-diamidino-2-phenylindol (DAPI) and visualized by EFM as well as AFM. The best imaging conditions for AFM were assessed.

Creative Commons License

Downloads

Download data is not yet available.

References

[1]. Q. Zhao, C. Wang, Y. Liu, S. Wang, Bacterial Adhesion on the Metal- Polymer Composite Coatings, International Journal of Adhesion and Adhesives, vol. 27, issue 2, p.85- 91, 2007.
[2]. D. Ornek, T.K. Wood, C.H. Hsu, Z. Sun, F. Mansfeld, Pitting Corrosion Control of Aluminum 2024 Using Protective Biofilms That Secrete Corrosion Inhibitors, Corrosion, vol. 58, issue 9, p. 761-768, 2002.
[3]. F. Mansfeld, H. Hsu, D. Ornek, T.K. Wood, B.C. Syrett, Corrosion Control Using Regenerative Biofilms on Aluminum 2024 and Brass in Different Media, Journal of the Electrochemical Society, vol. 149, issue 4, p. B130 –B138, 2002.
[4]. G. Gunasekaran, S. Chongdar, S.N. Gaonkar, P. Kumar, Influence of bacteria on film formation inhibiting corrosion, Corrosion Science, vol. 46, issue 8, p. 1953-1967, 2004.
[5]. I. B. Beech, J. A. Sunner, K. Hiraoka, Microbe-surface interactions in biofouling and biocorrosion processes, International Microbiology, vol. 8, issue 3, p. 157 - 168, 2005.
[6]. L. L. Barton, Sulphate-reducing bacteria, Plenum Press, New York, 1995.
[7]. R. Javaherdashti, A review of some characteristics of MIC caused by sulfate-reducing bacteria: past, present and future, Anti – Corrosion Method and Materials, vol. 46, issue 3, p. 173-180, 1999.
[8]. H. C. Flemming and J. Wingender, Relevance of microbial extracellular polymeric substances (EPSs) - Part II: Technical aspects, Water Science Technology, vol. 43, issue 6, p. 9-16, 2001.
[9]. W. Zhang, A. G. Stack, Y. Chen, Interaction force measurement between E. coli cells and nanoparticles immobilized surfaces by using AFM, Colloids and Surfaces B: Biointerfaces, vol. 82, issue 2, p. 316-324, 2011.
[10]. L. Kailas, E.C. Ratcliffe, E.J. Hayhurst, M.G. Walker, S.J. Foster, J.K. Hobbs, Immobilizing live bacteria for AFM imaging of cellular processes, Ultramicroscopy, vol. 109, issue 7, p. 775-780, 2009.
[11]. J. Li, J. Li, W. Yuan, Y. Du, Biocorrosion characteristics of the copper alloys BFe30-1-1 and HSn70-1AB by SRB using Atomic Force Microscopy and Scanning Electron Microscopy, International Biodeterioration & Biodegradation, vol. 64, issue 5, p. 363-370, 2010.
[12]. X. Sheng, Y. P. Ting, S. O. Pehkonen, The influence of sulphate-reducing bacteria biofilm on the corrosion of stainless steel AISI 316, Corrosion Science, vol. 49, issue 5, p. 2159-2176, 2007.
[13]. C. Xua, Y. Zhanga, G. Chenga, W. Zhu, Pitting corrosion behavior of 316L stainless steel in the media of sulphate-reducing and iron-oxidizing bacteria, Materials Characterization, vol. 59, issue 3, p. 245-255, 2008.
[14]. F. Kuang, J. Wang, L. Yana, D. Zhang, Effects of sulfatereducing bacteria on the corrosion behavior of carbon steel, Electrochimica Acta, vol. 52, issue 10, p. 6084-6088, 2007.
[15]. Jie Wen, Kaili Zhao, Tingyue Gu, Issam I. Raad, A green biocide enhancer for the treatment of sulfate-reducing bacteria (SRB) biofilms on carbon steel surfaces using glutaraldehyde, International Biodeterioration & Biodegradation, vol. 63, issue 8, p. 1102-1106, 2009.
[16]. D. G. Enos and S. R. Taylor, Influence of Sulfate-Reducing Bacteria on Alloy 625 and Austenitic Stainless Steel Weldments, Corrosion, vol.52, issue 11, p. 831-843, 1996.
[17]. Y. Wan, D. Zhang, H. Liu, Y. Li and B. Hou, Influence of sulphate-reducing bacteria on environmental parameters and marine corrosion behavior of Q235 steel in aerobic conditions, Electrochimica Acta, vol. 55, issue 5, p. 1528-1534, 2010.
[18]. J. Liu, X. Liang and S. Li, Effect of sulphate-reducing bacteria on the electrochemical impedance spectroscopy characteristics of 1Cr18Ni9Ti, Journal of University of Science and Technology Beijing, vol. 14, issue 5, p. 425-430, 2007.
[19]. T.S. Rao, Aruna Jyothi Kora, B. Anupkumar, S.V. Narasimhan, R. Feser, Pitting corrosion of titanium by a freshwater strain of sulphate reducing bacteria (Desulfovibrio vulgaris), Corrosion Science, vol. 47, issue 5, p.1071-1084, 2005.
[20]. H. Wanga, Z. Wang, H. Honga, Y. Yina, Preparation of cerium-doped TiO2 film on 304 stainless steel and its bactericidal effect in the presence of sulfate-reducing bacteria (SRB), Materials Chemistry and Physics, vol. 124, issue 1, p. 791-794, 2010.
[21]. O. Basu; S. A. Baldwin, Attachment and Growth of SulphateReducing Bacteria on Different Support Materials, Environmental Technology, vol. 21, issue 11, p. 1293-1300, 2000.
[22]. A.J. Silva, J.S. Hirasawa, M.B. Varesche, E. Foresti, M. Zaiat, Evaluation of support materials for the immobilization of sulfate-reducing bacteria and methanogenic archaea, Anaerobe, vol. 12, issue 2, p. 93-98, 2006.
Published
2011-03-15
How to Cite
1.
CIUBOTARIU A, BENEA L, SAND W. Sulphate Reducing Bacteria in Biofilms on Thermosetting Polymers/Zn Composite Layers. The Annals of “Dunarea de Jos” University of Galati. Fascicle IX, Metallurgy and Materials Science [Internet]. 15Mar.2011 [cited 28Mar.2024];34(1):51-6. Available from: https://www.gup.ugal.ro/ugaljournals/index.php/mms/article/view/2967
Section
Articles

Most read articles by the same author(s)

1 2 3 > >>